| Virus
Survival in Stool and Urine |
- Virus
is stable in faeces(and urine) at room temperature for at least
1-2 days.
|
- Virus
is more stable (up to 4 days) in stool from diarrhea patients
(which has higher pH than normal stool).
|
| |
|
| |
Disinfectants
and fixatives (for use in laboratories) |
| |
- Virus
loses infectivity after exposure to different commonly used disinfectants
and fixatives.
|
| |
|
|
| |
Virus
survival in cell-culture supernatant |
| |
- Only
minimal reduction in virus concentration after 21 days at 4°C
and -80°C.
|
| |
- Reduction
in virus concentration by one log only at stable room temperature
for 2 days. This would indicate that the virus is more stable
than the known human coronaviruses under these conditions.
|
| |
- Heat
at 56°C kills the SARS coronavirus at around 10000 units per
15 min (quick reduction).
|
| |
|
Lab* |
Substrate |
Initial
viral count log10PFU |
Condition |
Survival
time |
Method
of testing viability |
| GVU |
virus
spiked in baby stool |
1.00E+03
|
pH
6-7 |
3
hr |
Virus
isolation in cell culture |
| |
virus
spiked in normal stool |
1.00E+03
|
pH
8 |
6
hr |
Virus
isolation in cell culture |
| |
vvirus
in diarrheal stool |
1.00E+03
|
pH
9 |
4
days |
Virus
isolation in cell culture |
| QMH |
stool |
1.00E+03
|
Room
Temperature |
at
least 2 days |
Virus
isolation in cell culture |
| |
urine |
1.00E+03
|
Room
Temperature |
at
least 24 hr |
Virus
isolation in cell culture |
| |
Virus
culture medium+ 1% bovine serum |
1.00E+04
|
on
plastic surface in room temperature |
at
least 2 days |
Virus
isolation in cell culture |
| |
Virus
culture medium+ 1% bovine serum |
1.00E+04
|
30-37°C
|
at
least 1hr |
Virus
isolation in cell culture |
| |
Virus
culture medium+ 1% fetal calf serum |
1.00E+06 |
56°C
|
degration
of titre over time (10 000 infectious virus units in 15 min) |
Virus
isolation in cell culture |
| |
virus
in Acetone, 10% Formaldehyde and Paraformaldehyde, 10% Clorox, 75%ethanol,
2% phenol |
1.00E+06 |
Room
Temperature |
less
than 5 min |
Virus
isolation in cell culture |
| NIID |
Virus
culture+ 2% bovine serum |
1.00E+06 |
minus
80°C |
at
least 4 days |
Virus
isolation and RT-PCR |
| |
Virus
culture+ 2% fetal calf serum |
1.00E+06 |
4°C
|
at
least 4 days |
Virus
isolation and RT-PCR |
| |
Virus
culture+ 2% fetal calf serum |
1.00E+06 |
37°C
|
less
than 4 days |
Virus
isolation and RT-PCR |
| |
Virus
culture+ 2% fetal calf serum |
1.00E+05 |
56°C
|
less
than 30min |
|
| UniM |
Virus
culture |
1.00E+06 |
4°C
|
at
least 21 days |
Virus
isolation |
| |
Virus
culture |
1.00E+06
|
minus
80°C |
at
least 21 days |
Virus
isolation |
